abstract：:Despite over 30 years of work, the fundamental structure of eukaryotic chromatin remains controversial. Here, we review the roots of this controversy in disparities between results derived from studies of chromatin in nuclei, chromatin isolated from nuclei, and chromatin reconstituted from defined components. Thanks t...

journal_title：Experimental cell research

authors： Grigoryev SA,Woodcock CL

更新日期：2012-07-15 00:00:00

abstract：:Recent studies report mitochondrial carrier homolog 2 (MTCH2) as a novel and uncharacterized protein that acts as a receptor-like protein for the truncated BH3-interacting domain death agonist (tBID) protein in the outer membrane of mitochondria. These studies, using mouse embryonic stem cells and fibroblasts as well ...

journal_title：Experimental cell research

authors： Robinson AJ,Kunji ER,Gross A

更新日期：2012-07-01 00:00:00

abstract：:Over the last two decades molecular studies of inherited tumor syndromes that are associated with the development of kidney cancer have led to the identification of genes and biochemical pathways, which play key roles in the malignant transformation of renal epithelial cells. Some of these findings have broad biologic...

journal_title：Experimental cell research

authors： Haase VH

更新日期：2012-05-15 00:00:00

abstract：:The glomerular capillary endothelium is highly specialized to support the selective filtration of massive volumes of plasma. Filtration is driven by Starling forces acting across the glomerular capillary wall, and depends on its large surface area and extremely high water permeability. Glomerular endothelial cells are...

journal_title：Experimental cell research

authors： Obeidat M,Obeidat M,Ballermann BJ

更新日期：2012-05-15 00:00:00

abstract：:The kidney's glomerular filtration barrier consists of two cells-podocytes and endothelial cells-and the glomerular basement membrane (GBM), a specialized extracellular matrix that lies between them. Like all basement membranes, the GBM consists mainly of laminin, type IV collagen, nidogen, and heparan sulfate proteog...

journal_title：Experimental cell research

authors： Miner JH

更新日期：2012-05-15 00:00:00

abstract：:Three clonal subpopulations of DLKP, a poorly differentiated squamous lung carcinoma cell line, display striking differences in ability to survive in suspension (anoikis resistance). DLKP-SQ is anoikis resistant (7.5% anoikis at 24 h). In contrast, DLKP-M and DLKP-I are sensitive to anoikis (49.2% and 42.6% respective...

journal_title：Experimental cell research

authors： Keenan J,Joyce H,Aherne S,O'Dea S,Doolan P,Lynch V,Clynes M

更新日期：2012-03-10 00:00:00

abstract：:There is at present a controversy in the literature whether MSCs are susceptible to spontaneous in vitro transformation or not. Several groups have reported spontaneous transformation of MSCs from various species. However, some of these reports were not true transformations and later proven to be due to cross-contamin...

journal_title：Experimental cell research

authors： Torsvik A,Røsland GV,Bjerkvig R

更新日期：2012-03-10 00:00:00

abstract：:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

journal_title：Experimental cell research

authors： Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

更新日期：2012-03-10 00:00:00

abstract：OBJECTIVE:To investigate whether transmyocardial drilling revascularization combined with heparinized basic fibroblast growth factor (bFGF)-incorporating degradable stent implantation (TMDRSI) can promote myocardial regeneration after acute myocardial infarction (AMI). METHODS:A model of AMI was generated by ligating ...

journal_title：Experimental cell research

authors： Zhang GW,Wen T,Gu TX,Li-Ling J,Wang C,Zhao Y,Liu J,Wang Y,Liu TJ,Lü F

更新日期：2012-02-15 00:00:00

abstract：BACKGROUND:Functional activation of oncogenic K-Ras signaling pathway plays an important role in the early events of colorectal carcinogenesis (CRC). K-Ras proto-oncogene is involved in 35-40% of CRC cases. Mutations in the Ras gene trigger the transduction of proliferative and anti-apoptotic signals, even in the absen...

journal_title：Experimental cell research

authors： Naumov I,Kazanov D,Lisiansky V,Starr A,Aroch I,Shapira S,Kraus S,Arber N

更新日期：2012-01-15 00:00:00

abstract：:Chronic high glucose levels lead to the formation of advanced glycation end-products (AGEs) as well as AGE precursors, such as methylglyoxal (MG) and glyoxal, via non-enzymatic glycation reactions in patients with diabetic mellitus. Glyoxalase 1 (GLO-1) detoxifies reactive dicarbonyls that form AGEs. To investigate th...

journal_title：Experimental cell research

authors： Kim KM,Kim YS,Jung DH,Lee J,Kim JS

更新日期：2012-01-15 00:00:00

abstract：:Lamin A is a component of the nuclear envelope that is synthesized as a precursor prelamin A molecule and then processed into mature lamin A through sequential steps of posttranslational modifications and proteolytic cleavages. Remarkably, over 400 distinct point mutations have been so far identified throughout the LM...

journal_title：Experimental cell research

authors： Reddy S,Comai L

更新日期：2012-01-01 00:00:00

abstract：:Increased protein aggregation and altered cell signaling accompany many neurodegenerative diseases including Huntington's disease (HD). Cell stress is counterbalanced by signals mediating cell repair but the identity of these are not fully understood. We show here that the mammalian target of rapamycin (mTOR) pathway ...

journal_title：Experimental cell research

authors： Hyrskyluoto A,Reijonen S,Kivinen J,Lindholm D,Korhonen L

更新日期：2012-01-01 00:00:00

abstract：:The assumption that cells are temporally organized systems, i.e. showing relevant dynamics of their state variables such as gene expression or protein and metabolite concentration, while tacitly given for granted at the molecular level, is not explicitly taken into account when interpreting biological experimental dat...

journal_title：Experimental cell research

authors： Romagnoli G,Cundari E,Negri R,Crescenzi M,Farina L,Giuliani A,Bianchi MM

更新日期：2011-12-10 00:00:00

abstract：:RhoA activated kinases (ROCKs) are potent effectors of RhoA signaling for regulation of the cytoskeleton. ROCKs have been shown to be localized to several different subcellular locations, suggesting that its localization is context specific and regulated. However, the signaling mechanisms that control ROCK localizatio...

journal_title：Experimental cell research

authors： Kher SS,Worthylake RA

更新日期：2011-12-10 00:00:00

abstract：:The MUC6 mucin has a critical protective function in the normal stomach, pancreas and duodenum and is aberrantly expressed during the progression of some gastrointestinal cancers. Our aim was to determine whether MUC6 contributes to the etiology or progression of pancreatic cancer and elucidate the molecular basis of ...

journal_title：Experimental cell research

authors： Leir SH,Harris A

更新日期：2011-10-15 00:00:00

abstract：:The ChlR1 DNA helicase, encoded by DDX11 gene, which is responsible for Warsaw breakage syndrome (WABS), has a role in sister-chromatid cohesion. In this study, we show that human ChlR1 deficient cells exhibit abnormal heterochromatin organization. While constitutive heterochromatin is discretely localized at perinucl...

journal_title：Experimental cell research

authors： Inoue A,Hyle J,Lechner MS,Lahti JM

更新日期：2011-10-15 00:00:00

abstract：:Notch signaling involves the proteolytic cleavage of the transmembrane Notch receptor after binding to its transmembrane ligands. Jagged-1 also undergoes proteolytic cleavage by gamma-secretase and releases an intracellular fragment. In this study, we have demonstrated that the Jagged-1 intracellular domain (JICD) inh...

journal_title：Experimental cell research

authors： Kim MY,Jung J,Mo JS,Ann EJ,Ahn JS,Yoon JH,Park HS

更新日期：2011-10-15 00:00:00

abstract：OBJECTIVES:Our purpose was to investigate the role of the nitric oxide (NO) signaling pathway in the regulation of ciliary beat frequency (CBF) in mouse nasal and tracheal epithelial cells. METHODS:We studied the effects of the NO donor l-arginine (L-Arg) and specific inhibitors of the NO signaling pathway on CBF of b...

journal_title：Experimental cell research

authors： Jiao J,Wang H,Lou W,Jin S,Fan E,Li Y,Han D,Zhang L

更新日期：2011-10-15 00:00:00

abstract：:Endocytosis and transport of bovine liver β-glucuronidase to lysosomes in human fibroblasts are mediated by two receptors: the well-characterized cation-independent mannose 6-phosphate receptor (IGF-II/Man6PR) and an IGF-II/Man6PR-independent receptor, which recognizes a Ser-Trp*-Ser sequence present on the ligand. Th...

journal_title：Experimental cell research

authors： Ramírez-Mata A,Michalak C,Mendoza-Hernández G,León-Del-Río A,González-Noriega A

更新日期：2011-10-01 00:00:00

abstract：:The voltage-gated potassium channel, Kv1.3, plays an important role in regulating membrane excitability in diverse cell types ranging from T-lymphocytes to neurons. In the present study, we test the hypothesis that the C-terminal PDZ binding domain modulates the function and localization of Kv1.3. We created a mutant ...

journal_title：Experimental cell research

authors： Doczi MA,Damon DH,Morielli AD

更新日期：2011-10-01 00:00:00

abstract：:Bone morphogenetic proteins (BMPs) are multifunctional signaling molecules that have gained increasing interest in cancer research. To obtain a systematic view on BMP signaling in pancreatic cancer we first determined the mRNA expression levels of seven BMP ligands (BMP2-BMP8) and six BMP specific receptors in pancrea...

journal_title：Experimental cell research

authors： Virtanen S,Alarmo EL,Sandström S,Ampuja M,Kallioniemi A

更新日期：2011-09-10 00:00:00

abstract：:The extracellular matrix (ECM) contains important cues for tissue homeostasis and morphogenesis. The matricellular protein tenascin-C (TN-C) is overexpressed in remodeling tissues and cancer. In the present work, we studied the effect of different ECM-which exhibited a significant diversity in their TN-C content-in en...

journal_title：Experimental cell research

authors： Alves TR,da Fonseca AC,Nunes SS,da Silva AO,Dubois LG,Faria J,Kahn SA,Viana NB,Marcondes J,Legrand C,Moura-Neto V,Morandi V

更新日期：2011-09-10 00:00:00

abstract：:Gold nanoparticles are recently having much attention because of their increased applications in biomedical fields. In this paper, we demonstrated the photothermal efficacy of citrate capped gold nanoparticles (AuNPs) for the destruction of A431 cancer cells. Citrate capped AuNPs were synthesized successfully and char...

journal_title：Experimental cell research

authors： Raji V,Kumar J,Rejiya CS,Vibin M,Shenoi VN,Abraham A

更新日期：2011-08-15 00:00:00

abstract：:Pluripotent cells referred to as embryonic germ cells (EGCs) can be derived from the embryonic precursors of the mature gametes: the primordial germ cells (PGCs). A homozygous mutation (ter) of the dead-end homolog 1 gene (Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to neoplastic transformat...

journal_title：Experimental cell research

authors： Northrup E,Eisenblätter R,Glage S,Rudolph C,Dorsch M,Schlegelberger B,Hedrich HJ,Zschemisch NH

更新日期：2011-08-01 00:00:00

abstract：:The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knoc...

journal_title：Experimental cell research

authors： Haddad O,Gumez L,Hawse JR,Subramaniam M,Spelsberg TC,Bensamoun SF

更新日期：2011-07-15 00:00:00

abstract：:Mitochondrial DNA depleted (ρ(0)) human skin fibroblasts (HSF) with suppressed oxidative phosphorylation were characterized by significant changes in the expression of 2100 nuclear genes, encoding numerous protein classes, in NF-κB and STAT3 signaling pathways, and by decreased activity of mitochondrial death pathway,...

journal_title：Experimental cell research

authors： Ivanov VN,Ghandhi SA,Zhou H,Huang SX,Chai Y,Amundson SA,Hei TK

更新日期：2011-07-01 00:00:00

abstract：:Spermatogonial stem cells (SSCs) support life-long spermatogenesis by self-renewing and producing spermatogonia committed to differentiation. In vitro, SSCs form three-dimensional spermatogonial aggregates (clusters) when cultured with glial cell line-derived neurotrophic factor (GDNF) and fibroblast growth factor 2 (...

journal_title：Experimental cell research

authors： Ebata KT,Yeh JR,Zhang X,Nagano MC

更新日期：2011-06-10 00:00:00

abstract：:Interferon beta is widely used as first-line treatment for relapsing remitting multiple sclerosis (RRMS). Several products are marketed world-wide, and biosimilar products are emerging. Interferon beta reduces relapse rates by about 1/3, and reduces the appearance of new MRI lesions by about 2/3, and some studies have...

journal_title：Experimental cell research

authors： Rudick RA,Goelz SE

更新日期：2011-05-15 00:00:00

abstract：:Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/we...

journal_title：Experimental cell research

authors： Oikawa Y,Hansson J,Sasaki T,Rousselle P,Domogatskaya A,Rodin S,Tryggvason K,Patarroyo M

更新日期：2011-05-01 00:00:00

abstract：:Mutations in PKHD1 (polycystic kidney and hepatic disease gene 1) gene cause the autosomal recessive polycystic kidney disease (ARPKD). Fibrocystin/polyductin (FPC), encoded by PKHD1, is a membrane-associated receptor-like protein. Although it is widely accepted that cystogenesis is mostly due to aberrant cell prolife...

journal_title：Experimental cell research

authors： Sun L,Wang S,Hu C,Zhang X

更新日期：2011-04-15 00:00:00

abstract：:The extraocular muscles (EOM) are spared from pathology in aging and many forms of muscular dystrophy. Despite many studies, this sparing remains an enigma. The EOM have a distinct embryonic lineage compared to somite-derived muscles, and we have shown that they continuously remodel throughout life, maintaining a popu...

journal_title：Experimental cell research

authors： Kallestad KM,Hebert SL,McDonald AA,Daniel ML,Cu SR,McLoon LK

更新日期：2011-04-01 00:00:00

abstract：:Abscission marks the completion of cell division and its failure is associated with delayed cytokinesis and even tetraploidization. Aberrant abscission and consequential ploidy changes can underlie various diseases including cancer. Midbody, a transient structure formed in the intercellular bridge during telophase, co...

journal_title：Experimental cell research

authors： Singh U,Westermark B

更新日期：2011-01-15 00:00:00

abstract：:Tumor angiogenesis and immune response have in common to be cell recognition mechanisms, which are based on specific adhesion molecules and dependent on nitric oxide (NO(•)). The aim of the present study is to deepen the mechanisms of angiogenesis and inflammation regulation by NO(•) to find out the molecular regulati...

journal_title：Experimental cell research

authors： Carreau A,Kieda C,Grillon C

更新日期：2011-01-01 00:00:00

abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

journal_title：Experimental cell research

authors： Zhang J,Harrison JS,Studzinski GP

更新日期：2011-01-01 00:00:00

abstract：:Accumulating evidence shows that the inhibition of thromboxane synthase (TXS) induced apoptosis in cancer cells. TXS inhibitor 1-Benzylimidzole (1-BI) can trigger apoptosis in lung cancer cells but the mechanism is not fully defined. In this study, lung cancer cells were treated with 1-BI. In this study, the level of ...

journal_title：Experimental cell research

authors： Leung KC,Li MY,Leung BC,Hsin MK,Mok TS,Underwood MJ,Chen GG

更新日期：2010-12-10 00:00:00

abstract：BACKGROUND AND AIMS:B-cell lymphoma/leukemia (BCL)-10 and reactive oxygen species mediate two pathways of NF-κB (RelA) activation by lipopolysaccharide (LPS) in human colonic epithelial cells. The pathway for LPS activation of RelB by the non-canonical pathway (RelB) in non-myeloid cells was not yet reported, but impor...

journal_title：Experimental cell research

authors： Bhattacharyya S,Borthakur A,Dudeja PK,Tobacman JK

更新日期：2010-11-15 00:00:00

abstract：:Growth and survival of tumors at a site of metastasis involve interactions with stromal cells in the surrounding environment. Stromal cells aid tumor cell growth by producing cytokines as well as by modifying the environment surrounding the tumor through modulation of the extracellular matrix (ECM). Small leucine-rich...

journal_title：Experimental cell research

authors： Coulson-Thomas VJ,Gesteira TF,Coulson-Thomas YM,Vicente CM,Tersariol IL,Nader HB,Toma L

更新日期：2010-11-15 00:00:00

abstract：:With the aim of discovering new molecular interactions of the tight junction protein ZO-2, a two-hybrid screen was performed on a human kidney cDNA library using as bait the middle segment of ZO-2. Through this assay we identified a 24-kDa novel protein herein named ZASP for ZO-2 associated speckle protein. ZO-2/ZASP ...

journal_title：Experimental cell research

authors： Lechuga S,Alarcón L,Solano J,Huerta M,Lopez-Bayghen E,González-Mariscal L

更新日期：2010-11-15 00:00:00

abstract：:Skeletal muscles cope with a large range of activities, from being able to support the body weight during long periods of upright standing to perform explosive movements in response to an unexpected threat. This requires systems for energy metabolism that can provide energy during long periods of moderately increased ...

journal_title：Experimental cell research

authors： Westerblad H,Bruton JD,Katz A

更新日期：2010-11-01 00:00:00